Six wild-caught, captive canyon tree frogs (Hyla arenicolor) purchased as a group and housed at the North Carolina Zoological Park were diagnosed with intradermal Hannemania mites, the first described infestation of this frog species by this mite. Typical gross lesions were orange-colored skin vesicles approximately 1 mm in diameter, predominantly located on the ventrum and ventral hind limbs. The larval mites had ovoid bodies approximately 44 µm in length and 240 µm in width. Three of the frogs died, and three became free of mites and pathologic changes after approximately 28 wk of treatment with transcutaneous ivermectin and manual mite removal. The frogs remained free of mites at a 1-yr recheck.